Functional and Structural Characterization of Neutralizing Epitopes of Measles Virus Hemagglutinin Protein

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Functional and structural characterization of neutralizing epitopes of measles virus hemagglutinin protein.

Effective vaccination programs have dramatically reduced the number of measles-related deaths globally. Although all the available data suggest that measles eradication is biologically feasible, a structural and biochemical basis for the single serotype nature of measles virus (MV) remains to be provided. The hemagglutinin (H) protein, which binds to two discrete proteinaceous receptors, is the...

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Measles Virus Hemagglutinin Protein Epitopes: The Basis of Antigenic Stability

Globally eliminating measles using available vaccines is biologically feasible because the measles virus (MV) hemagglutinin (H) protein is antigenically stable. The H protein is responsible for receptor binding, and is the main target of neutralizing antibodies. The immunodominant epitope, known as the hemagglutinating and noose epitope, is located near the receptor-binding site (RBS). The RBS ...

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Identification of immunodominant neutralizing epitopes on the hemagglutinin protein of rinderpest virus.

The immunodominant epitopes on the hemagglutinin protein of rinderpest virus (RPV-H) were determined by analyzing selected monoclonal antibody (MAb)-resistant mutants and estimating the level of antibody against each epitope in five RPV-infected rabbits with the competitive enzyme-linked immunosorbent assay (c-ELISA). Six neutralizing epitopes were identified, at residues 474 (epitope A), 243 (...

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The receptor-binding site of the measles virus hemagglutinin protein itself constitutes a conserved neutralizing epitope.

Here, we provide direct evidence that the receptor-binding site of measles virus (MV) hemagglutinin protein itself forms an effective conserved neutralizing epitope (CNE). Several receptor-interacting residues constitute the CNE. Thus, viral escape from neutralization has to be associated with loss of receptor-binding activity. Since interactions with both the signaling lymphocyte activation mo...

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Designing of A Multi-epitope Recombinant Protein, Consisting of Several Conserved Epitopes from Hemagglutinin Protein of the H1N1 and H5N1 Strains of Influenza Virus by Immunoinformatics Approaches

Introduction: According to marked advances in bioinformatics studies, development of influenza vaccines has been greatly modified in many studies. In this study, we have designed a multi-epitope recombinant protein, consisting of several conserved epitopes from Hemagglutinin protein of the H1N1 and H5N1 strains of Influenza virus by immunoinformatics approaches. Materials and Methods: The regis...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 2012

ISSN: 0022-538X

DOI: 10.1128/jvi.02033-12